PBMC Therapy

Rejuvenating Ovary & Uterine Lining
Innovative solution for women’s infertility

PBMC Therapy

PERIPHERAL BLOOD MONONUCLEAR CELLS IMMUNOTHERAPY (PBMC) Successful implantation depends on local maternal immune tolerance. It is hypothesized that patients with RIF may be unable to recruit the necessary lymphocytes needed for successful implantation but that intrauterine infusion of a patient’s own lymphocytes may restore the immune balance and improve endometrial receptivity and implantation rates. Peripheral blood mononuclear cells (PBMCs) contain T and B lymphocytes and monocytes. These cells induce the production of cytokines, interleukins, and growth factors, which has been shown in some studies to exert a positive effect on the endometrial thickness and endometrial receptivity. Implantation failure is a major limiting factor in assisted reproduction improvement. Dysfunction of embryo-maternal Immuno-tolerance pathways may be responsible for repeated implantation failures.

Flow Chart

i. BLOOD COLLECTION (from the women patients)
ii. PREPARATION OF PBMC ( Laboratory procedure)
PRP Thera

Procedure of Intrauterine Infusion

  • • After antagonist ovarian stimulation protocol,
  • The blood sample is scheduled on a specific day to isolate PBMCs
  • PBMCs prepared in Laboratory
  • Then PBMC will transfer inside the uterine cavity with the help of an ET catheter two days before embryo transfer.
  • After embryo transfer, patients receive oral Utrogestan (Micronised Progesterone) 200 mg_2/day for luteal support.


    • Turocy, J., & Williams, Z. (2021). Novel therapeutic options for treatment of recurrent implantation failure. Fertility and sterility, 116(6), 1449-1454.

    • Sefrioui, O., Madkour, A., Bouamoud, N., Kaarouch, I., Saadani, B., Amzazi, S., … & with the Lorem Ipsum Consortium. (2019). Luteal progesterone level correlated with immunotherapy success of patients with repeated implantation failures. BioRxiv, 603720.

    • Yu, N., Zhang, B., Xu, M., Wang, S., Liu, R., Wu, J., … & Feng, L. (2016). Intrauterine administration of autologous peripheral blood mononuclear cells (PBMC s) activated by HCG improves the implantation and pregnancy rates in patients with repeated implantation failure: a prospective randomized study. American Journal of Reproductive Immunology, 76(3), 212-216.

    • Madkour, A., Bouamoud, N., Louanjli, N., Kaarouch, I., Copin, H., Benkhalifa, M., & Sefrioui, O. (2016). Intrauterine insemination of cultured peripheral blood mononuclear cells prior to embryo transfer improves clinical outcome for patients with repeated implantation failures. Zygote, 24(1), 58-69.

    • Fujiwara, H. (2006). Immune cells contribute to systemic cross‐talk between the embryo and mother during early pregnancy in cooperation with the endocrine system. Reproductive medicine and biology, 5(1), 19-29.